Dr. Julian Wolf

Curriculum vitae

Dr. med. Julian Wolf, MD




Killianstraße 5
79106 Freiburg im Breisgau


Professional experience

03/2022 - present:

Research fellow, Department of Ophthalmology, Stanford University, Palo Alto, CA

03/2019 - present:

Residency, University Eye Hospital Freiburg, Germany

01/2019 – 02/2019:

Retina Service, New York Eye and Ear Infirmary, Prof. Richard Rosen, New York City, United States

Academic qualifications

03/2022 - present:

Postdoctoral scholarship, Omics Laboratory, Department of Ophthalmology, Byers Eye Institute, Stanford University, Palo Alto, CA, Prof. V. Mahajan


Dr. med., University of Bochum, Germany


German Medical License Examination (sehr gut)

2012 - 2018:

Medical school, University of Bochum, Germany
Clinical electives at University Eye Hospital Freiburg, Germany and Waidspital Zurich, Switzerland



Childrens’ Eye Cancer Foundation Germany


Volker Homann Foundation (with Prof. Lange)



Henning-Zügel-Stiftung Research Award, University of Freiburg


Two best presentation awards, German Society for Ophthalmology




Wolf J, Boneva S, Rosmus DD, Agostini H, Schlunck G, Wieghofer P, Schlecht A, Lange C. In-Depth Molecular Profiling Specifies Human Retinal Microglia Identity. Front Immunol. 2022 Mar 18;13:863158. doi: 10.3389/fimmu.2022.863158. PMID: 35371110.


Wolf J, Boneva S, Rosmus DD, Agostini H, Schlunck G, Wieghofer P, Schlecht A, Lange C. Deciphering the Molecular Signature of Human Hyalocytes in Relation to Other Innate Immune Cell Populations. Invest Ophthalmol Vis Sci. 2022 Mar 2;63(3):9. doi: 10.1167/iovs.63.3.9. PMID: 35266958.


Wolf J, Lapp T, Reinhard T, Agostini H, Schlunck G, Lange C. Webbasierte Genexpressionsanalysen – auf dem Weg zur molekularen Entschlüsselung gesunder und erkrankter Augengewebe [Web-based gene expression analysis-paving the way to decode healthy and diseased ocular tissue]. Ophthalmologe. 2022 Feb 22:1–8. German. doi: 10.1007/s00347-022-01592-9. Epub ahead of print. PMID: 35194679.


Wolf J, Boneva S, Schlecht A, Lapp T, Auw-Haedrich C, Lagrèze W, Agostini H, Reinhard T, Schlunck G, Lange C. The Human Eye Transcriptome Atlas: A searchable comparative transcriptome database for healthy and diseased human eye tissue. Genomics. 2022 Feb 3:110286. doi: 10.1016/j.ygeno.2022.110286. Epub ahead of print. PMID: 35124170.


Wolf J, Hajdu RI, Boneva S, Schlecht A, Lapp T, Wacker K, Agostini H, Reinhard T, Auw-Hädrich C, Schlunck G, Lange C. Characterization of the Cellular Microenvironment and Novel Specific Biomarkers in Pterygia Using RNA Sequencing. Front Med (Lausanne). 2022 Jan 31;8:714458. doi: 10.3389/fmed.2021.714458. PMID: 35174178.


Wolf J, Schlecht A, Rosmus DD, Boneva S, Agostini H, Schlunck G, Wieghofer P, Lange C. Comparative transcriptome analysis of human and murine choroidal neovascularization identifies fibroblast growth factor inducible-14 as phylogenetically conserved mediator of neovascular age-related macular degeneration. Biochim Biophys Acta Mol Basis Dis. 2022 Jan 12;1868(4):166340. doi: 10.1016/j.bbadis.2022.166340. Epub ahead of print. PMID: 35032596.



Schlecht A, Thien A, Wolf J, Prinz G, Agostini H, Schlunck G, Wieghofer P, Boneva S, Lange C. Immunosenescence in Choroidal Neovascularization (CNV)-Transcriptional Profiling of Naïve and CNV-Associated Retinal Myeloid Cells during Aging. Int J Mol Sci. 2021 Dec 10;22(24):13318. doi: 10.3390/ijms222413318. PMID: 34948115.


Boneva SK, Wolf J, Hajdú RI, Prinz G, Salié H, Schlecht A, Killmer S, Laich Y, Faatz H, Lommatzsch A, Busch M, Bucher F, Stahl A, Böhringer D, Bengsch B, Schlunck G, Agostini H, Lange CAK. In-Depth Molecular Characterization of Neovascular Membranes Suggests a Role for Hyalocyte-to-Myofibroblast Transdifferentiation in Proliferative Diabetic Retinopathy. Front Immunol. 2021 Nov 2;12:757607. doi: 10.3389/fimmu.2021.757607. PMID: 34795670.


Martin G, Wolf J, Lapp T, Agostini HT, Schlunck G, Auw-Hädrich C, Lange CAK. Viral S protein histochemistry reveals few potential SARS-CoV-2 entry sites in human ocular tissues. Sci Rep. 2021 Sep 27;11(1):19140. doi: 10.1038/s41598-021-98709-y. PMID: 34580409.


Schlecht A, Boneva S, Salie H, Killmer S, Wolf J, Hajdu RI, Auw-Haedrich C, Agostini H, Reinhard T, Schlunck G, Bengsch B, Lange CA. Imaging mass cytometry for high-dimensional tissue profiling in the eye. BMC Ophthalmol. 2021 Sep 20;21(1):338. doi: 10.1186/s12886-021-02099-8. PMID: 34544377.


Zhang P, Schlecht A, Wolf J, Boneva S, Laich Y, Koch J, Ludwig F, Boeck M, Thien A, Härdtner C, Kierdorf K, Agostini H, Schlunck G, Prinz M, Hilgendorf I, Wieghofer P, Lange C. The role of interferon regulatory factor 8 for retinal tissue homeostasis and development of choroidal neovascularisation. J Neuroinflammation. 2021 Sep 20;18(1):215. doi: 10.1186/s12974-021-02230-y. PMID: 34544421.


Tenbrock L, Wolf J, Boneva S, Schlecht A, Agostini H, Wieghofer P, Schlunck G, Lange C. Subretinal fibrosis in neovascular age-related macular degeneration: current concepts, therapeutic avenues, and future perspectives. Cell Tissue Res. 2021 Sep 3. doi: 10.1007/s00441-021-03514-8. Epub ahead of print. PMID: 34477966.


Zhuang X, Schlunck G, Wolf J, Rosmus DD, Bleul T, Luo R, Böhringer D, Wieghofer P, Lange C, Reinhard T, Lapp T. Time- and Stimulus-Dependent Characteristics of Innate Immune Cells in Organ-Cultured Human Corneal Tissue. J Innate Immun. 2021 Jun 28:1-14. doi: 10.1159/000516669. PMID: 34182556.


Lange C, Wolf J, Auw-Haedrich C, Schlecht A, Boneva S, Lapp T, Agostini H, Martin G, Reinhard T, Schlunck G. What is the significance of the conjunctiva as a potential transmission route for SARS-CoV-2 infections? Ophthalmologe. 2021 Jan;118(Suppl 1):85-88. doi: 10.1007/s00347-020-01255-7. PMID: 33141332.


Schlecht A, Zhang P, Wolf J, Thien A, Rosmus DD, Boneva S, Schlunck G, Lange C, Wieghofer P. Secreted Phosphoprotein 1 Expression in Retinal Mononuclear Phagocytes Links Murine to Human Choroidal Neovascularization. Front Cell Dev Biol. 2021 Jan 28;8:618598. doi: 10.3389/fcell.2020.618598. PMID: 33585455.



Boneva SK, Schlecht A, Zhang P, Boehringer D, Lapp T, Mittelviefhaus H, Reinhard T, Auw-Haedrich C, Schlunck G, Wolf J, Lange C. MACE RNA sequencing analysis of conjunctival squamous cell carcinoma and papilloma using formalin-fixed paraffin-embedded tumor tissue. Sci Rep. 2020 Dec 4;10(1):21292. doi: 10.1038/s41598-020-78339-6. PMID: 33277602.


Wolf J, Zhuang X, Hildebrand A, Boneva S, Schwämmle M, Kammrath Betancor P, Fan J, Böhringer D, Maier P, Lange C, Reinhard T, Schlunck G, Lapp T. Corneal tissue induces transcription of metallothioneins in monocyte-derived human macrophages. Mol Immunol. 2020 Oct 30;128:188-194. doi: 10.1016/j.molimm.2020.10.016. PMID: 33137607.


Wolf J, Auw-Haedrich C, Schlecht A, Boneva S, Mittelviefhaus H, Lapp T, Agostini H, Reinhard T, Schlunck G, Lange CAK. Transcriptional characterization of conjunctival melanoma identifies the cellular tumor microenvironment and prognostic gene signatures. Sci Rep. 2020 Oct 12;10(1):17022. doi: 10.1038/s41598-020-72864-0. PMID: 33046735.


Boneva SK, Wolf J, Rosmus DD, Schlecht A, Prinz G, Laich Y, Boeck M, Zhang P, Hilgendorf I, Stahl A, Reinhard T, Bainbridge J, Schlunck G, Agostini H, Wieghofer P, Lange CAK. Transcriptional Profiling Uncovers Human Hyalocytes as a Unique Innate Immune Cell Population. Front Immunol. 2020 Sep 11;11:567274. doi: 10.3389/fimmu.2020.567274. PMID: 33042148.


Lange C, Wolf J, Auw-Haedrich C, Schlecht A, Boneva S, Lapp T, Agostini H, Martin G, Reinhard T, Schlunck G. Welche Bedeutung hat die Bindehaut als möglicher Übertragungsweg für eine SARS-CoV-2-Infektion? [What is the importance of the conjunctiva as a potential transmission pathway for SARS-CoV-2 infections?]. Ophthalmologe. 2020 Jul;117(7):626-630. German. doi: 10.1007/s00347-020-01150-1. PMID: 32572552.


Schlunck G, Boneva S, Wolf J, Schlecht A, Reinhard T, Auw-Hädrich C, Lange C. RNA Sequencing of Formalin-Fixed and Paraffin-Embedded Tissue as a Complementary Method in Ophthalmopathology. Klin Monbl Augenheilkd. 2020 Jul;237(7):860-866. English, German. doi: 10.1055/a-1187-1590. Epub 2020 Jul 13. PMID: 32659839.


Boneva S, Schlecht A, Böhringer D, Mittelviefhaus H, Reinhard T, Agostini H, Auw-Haedrich C, Schlunck G, Wolf J*, Lange C*. 3' MACE RNA-sequencing allows for transcriptome profiling in human tissue samples after long-term storage. Lab Invest. 2020 May 28. doi: 10.1038/s41374-020-0446-z. Epub ahead of print. PMID: 32467590. * contributed equally as senior authors.


Lange C, Wolf J, Auw-Haedrich C, Schlecht A, Boneva S, Lapp T, Horres R, Agostini H, Martin G, Reinhard T, Schlunck G. Expression of the COVID-19 receptor ACE2 in the human conjunctiva. J Med Virol. 2020 May 6:10.1002/jmv.25981. doi: 10.1002/jmv.25981. Epub ahead of print. PMID: 32374427.


Schlecht A, Boneva S, Gruber M, Zhang P, Horres R, Bucher F, Auw-Haedrich C, Hansen L, Stahl A, Hilgendorf I, Agostini H, Wieghofer P, Schlunck G, Wolf J*, Lange CAK*. Transcriptomic Characterization of Human Choroidal Neovascular Membranes Identifies Calprotectin as a Novel Biomarker for Patients with Age-Related Macular Degeneration. Am J Pathol. 2020 Aug;190(8):1632-1642. doi: 10.1016/j.ajpath.2020.04.004. Epub 2020 Apr 24. PMID: 32339498. * contributed equally as senior authors.


Boeck M, Thien A, Wolf J, Hagemeyer N, Laich Y, Yusuf D, Backofen R, Zhang P, Boneva S, Stahl A, Hilgendorf I, Agostini H, Prinz M, Wieghofer P, Schlunck G, Schlecht A, Lange C. Temporospatial distribution and transcriptional profile of retinal microglia in the oxygen-induced retinopathy mouse model. Glia. 2020 Sep;68(9):1859-1873. doi: 10.1002/glia.23810. Epub 2020 Mar 9. PMID: 32150307.


Gruber M, Wolf J, Stahl A, Ness T, Scholl H, Agostini H, Maloca P, Lange C. Novel insights into retinal neovascularization secondary to central serous chorioretinopathy using 3D optical coherence tomography angiography. Am J Ophthalmol Case Rep. 2020 Feb 11;18:100609. doi: 0.1016/j.ajoc.2020.100609. PMID: 32123772.


Further qualifications


Good Clinical Practice (GCP) course


Course on laboratory animal science (FELASA B)

12/2017 - 01/2019:

Professional Certificate Program Data Science, Harvard University, Professional certificate earned on edx.

05/2018 - 07/2018:

Specialization Mastering Software Development in R, Johns Hopkins University, certificate earned on Coursera.


Writing in the Sciences, Stanford University, Certificate earned on Coursera.