Hyalocytes were FACS isolated from five enucleated eyes immediately after enucleation as well as from nine patients undergoing vitrectomy due to macular hole or macular pucker. Standard RNA-sequencing was applied for transcriptome analysis. Details regarding the isolation and sequencing protocol are provided in Boneva et al. (2020).
Fastq files of all replicates can be downloaded from European Nucleotide Archive using the following links. Please cite the publication shown above, when using data for your research.
| Sample | Age at surgery (years) | Sex | FFPE/unfixed | Fastq files |
|---|---|---|---|---|
| 1 (vitrectomy) | 72.0 | male | unfixed | flowcell 1, flowcell 2, flowcell 3 |
| 2 (vitrectomy) | 72.8 | male | unfixed | flowcell 1, flowcell 2, flowcell 3 |
| 3 (vitrectomy) | 87.2 | male | unfixed | flowcell 1, flowcell 2, flowcell 3 |
| 4 (vitrectomy) | 77.3 | female | unfixed | flowcell 1, flowcell 2, flowcell 3 |
| 5 (vitrectomy) | 69.0 | male | unfixed | flowcell 1, flowcell 2, flowcell 3 |
| 6 (vitrectomy) | 78.0 | female | unfixed | flowcell 1, flowcell 2, flowcell 3 |
| 7 (vitrectomy) | 72.1 | female | unfixed | flowcell 1, flowcell 2, flowcell 3 |
| 8 (vitrectomy) | 76.1 | female | unfixed | flowcell 1, flowcell 2, flowcell 3 |
| 9 (vitrectomy) | 67.1 | male | unfixed | flowcell 1, flowcell 2, flowcell 3 |
| 10 (enucleation) | 52.7 | female | unfixed | coming soon |
| 11 (enucleation) | 51.2 | male | unfixed | coming soon |
| 12 (enucleation) | 92.5 | female | unfixed | coming soon |
| 13 (enucleation) | 55.0 | male | unfixed | coming soon |
| 14 (enucleation) | 60.5 | male | unfixed | coming soon |