For RNA sequencing analysis of human CNV membranes we retrospectively analyzed four consecutive nAMD patients who underwent subretinal CNV extraction during vitreoretinal surgery between 1992 and 1999. All CNV membranes were treatment-naïve classic CNV and processed for formalin-fixation and paraffin-embedding (FFPE) immediately following extraction. Following a 20-gauge vitrectomy, we performed retinotomy temporal to the macula and surgically extracted the CNV membrane using a flexed forceps. The diagnosis was made after a thorough funduscopic examination of both eyes. Only patients exhibiting a CNV associated with AMD changes, such as drusen and RPE alterations, were included in the study. CNV membranes from patients with other diseases, such as myopia or a history of central serous chorioretinopathy, were not included in this study. The 3'-RNA-sequencing method MACE was applied for sequencing of FFPE samples, as described in Boneva et al. (2020).
Fastq files of all replicates can be downloaded from European Nucleotide Archive using the following links. Please cite the publication shown above, when using data for your research.